Journal: bioRxiv

Article Title: High speed functional imaging with a microfluidics-compatible open-top light-sheet microscope enabled by model predictive control of a tunable lens

doi: 10.1101/2025.07.23.666439

Figure Lengend Snippet: Open-top light-sheet microscope with electrically tunable lens (ETL) remote focusing under model predictive control. (a) Scan methods for volumetric light-sheet imaging. (i) In the simplest case, a sample is scanned through a static sheet. (ii) An actuated objective lens can follow a scanning light-sheet, which is faster than (i) but inertia limited and can cause problems with water immersion. (iii) Remote focusing optically shifts the focal plane using an active element like an ETL (b). This setup has less inertia and no moving parts at the sample. (c) Long-working distance open-top imaging is achieved using an asymmetric pair of objective lenses coupled with a water immersion fitting. This enables unobstructed imaging across a water-matched barrier such as FEP (above). (d) Maximum intensity projections along the Z (top) and Y (bottom) axes of a worm expressing a pan-neuronal nuclear-localized fluorescent protein positioned in a microfluidic channel as shown in (c). Scale bar 20 µ m. (e) Fast actuation of an ETL induces high frequency oscillation which slows response time. Performance is improved by using model predictive control to optimize drive signals. The controller iteratively optimizes the input signal to the ETL by minimizing simulated output error while obeying system constraints.

Article Snippet: Finally, we used the generated model to construct a model predictive controller using the MATLAB Model Predictive Control toolkit.

Techniques: Microscopy, Control, Imaging, Expressing